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KMID : 1134820140430091462
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 9 p.1462 ~ p.1466
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Optimization of Alkali Extraction for Preparing Oat Protein Concentrates from Oat Groat by Response Surface Methodology
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Jeong Yong-Seon
Kim Jeong-Won
Lee Eui-Seok
Gil Na-Young
Kim San-Seong
Hong Soon-Tack
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Abstract
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In this study, an attempt was made to produce oat protein concentrates from defatted oat groat by alkali extraction. Independent variables formulated by D-optimal design were NaOH concentration (X1, 0.005～0.06 N) for extraction and precipitation pH (X2, pH 4.0～6.0), and the dependent variable was extraction yield (Y1, %). Experimental results were analyzed by response surface methodology to determine optimized extraction conditions. Extraction yield increased both with an increase in NaOH concentration of the extraction solution and when approaching a precipitation pH of 4.9, and NaOH concentrations were a major influencing parameter. Solubility of oat protein concentrates showed a minimum value (i.e., 0.1%) at pH 5 and increased substantially at pH values in the range of ≤pH 3 or ≥pH 7, reaching a maximum value at pH 11 (i.e., 76%). Regression equation coincided well with the results of the experiment. Optimized extraction conditions to maximize extraction yield were 0.06 N NaOH (X1) for extraction and pH 4.7 (X2) for precipitation.
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KEYWORD
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oat protein concentrates, alkali extraction, optimization, nitrogen solubility index
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